Solution structure and dynamics of the bioactive retroviral M domain from rous sarcoma virus
Title | Solution structure and dynamics of the bioactive retroviral M domain from rous sarcoma virus |
Publication Type | Journal Articles |
Year of Publication | 1998 |
Authors | McDonnell JM, Fushman D, Cahill SM, Zhou W, Wolven A, Wilson CB, Nelle TD, Resh MD, Wills J, Cowburn D |
Journal | Journal of Molecular Biology |
Volume | 279 |
Issue | 4 |
Pagination | 921 - 928 |
Date Published | 1998/06/19/ |
ISBN Number | 0022-2836 |
Keywords | heteronuclear NMR spectroscopy, protein dynamics, RSV matrix protein, sequence homology, three-dimensional structure |
Abstract | A biologically active construct of the retroviral M domain from the avian Rous sarcoma virus is defined and its solution structure described. This M domain is fully active in budding and infectivity without myristylation. In spite of a sequence homology level that suggests no relationship among M domains and the family of matrix proteins in mammalian retroviruses, the conserved structural elements of a central core allow an M domain sequence motif to be described for all retroviruses. The surface of the M domain has a highly clustered positive patch comprised of sequentially distant residues. An analysis of the backbone dynamics, incorporating rotational anisotropy, is used to estimate the thermodynamics of proposed domain oligomerization. |
URL | http://www.sciencedirect.com/science/article/pii/S0022283698917880 |
DOI | 10.1006/jmbi.1998.1788 |